The purpose of the research was to find out newer antibiotics from Micromonospora species isolated from soil. Actinomycetes species is one of the important source of antibiotics. In spite of the many advances in microbiology, biochemistry, drug discovery and development in recent years, we are not keeping pace with the ability of microbes to adapt to and resist antibacterials. Considering the same this research work was taken up to screen newer antibiotic from soil. The Micromonospora species was obtained from soil collected from different location of Pune and Belgaum. For the isolation of the strain of Micromonospora, crowded plated technique and heat shock mathod was used. The isolate was characterized by studying its morphology , microscopy and also tested for the activity of secondary metabolites. The isolated antibiotic was found bactericidal against Gram negative microorganisms. Environmental parameter like temapature and pH optimization was done to get a maximum yield. The optimum temapareture set at 450C to 500C and pH was set at 6. Antibiotic was extracted from broth by Ethyl acetate. The MIC of the antibiotic obtained was determined against E. coli (0.9 µg/ml). Thin Layer Chromatography of the antibiotic was carried out. The antibiotic was subjected for spectroscopic analysis (UV, IR and NMR).
Loading....